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human ppp3 catalytic subunit alpha  (Santa Cruz Biotechnology)


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    Santa Cruz Biotechnology human ppp3 catalytic subunit alpha
    Obestatin promotes activation of NFTAc1. a Comparation of the effect of obestatin (10 nM) and insulin (1.72 µM) on utrophin, ß-dystroglycan, and α7-integrin protein expression on differentiating human DMD cells. b Analysis of pNFATc1(S172) and NFATc1 after obestatin (10 nM) or insulin (1.72 µM) administration on differentiating human DMD cells. c Representative immunofluorescence images of obestatin- or control-treated human DMD myotubes showing NFATc1 cellular location. Right panel , measurement of the number of nuclei showing NFATc1 location. Data are shown as mean ± SEM (* P < 0.05). d Representative images of vehicle- and obestatin-treated TAs showing NFATc1 expression. Right panel , measurement of the number of nuclei showing NFATc1 location. Data are shown as mean ± SEM (* P < 0.05). e Immunoblot analysis of <t>PPP3</t> catalytic subunit (PPP3CA), pNFATc1(S172), NFATc1, utrophin, slow-MHC, fast-MHC, β-dystroglycan, α-syntrophin, NOS1, β1D-integrin, and α7-integrin in extracts of DMD cells transfected with control or PPP3CA siRNAs after obestatin treatment (10 nM). The inset shows Proposed model by which obestatin signalling triggers PPP3 to regulate the transcriptional activity of NFATc1. Data were expressed as mean ± SEM ( n = 3 per group; *, # P < 0.05)
    Human Ppp3 Catalytic Subunit Alpha, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 92/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+ppp3+catalytic+subunit+alpha/pmc12647412-285-11-17?v=Santa+Cruz+Biotechnology
    Average 92 stars, based on 4 article reviews
    human ppp3 catalytic subunit alpha - by Bioz Stars, 2026-07
    92/100 stars

    Images

    1) Product Images from "Obestatin treatment links mitochondrial homeostasis and skeletal muscle repair in Duchenne muscle dystrophy"

    Article Title: Obestatin treatment links mitochondrial homeostasis and skeletal muscle repair in Duchenne muscle dystrophy

    Journal: Molecular Biomedicine

    doi: 10.1186/s43556-025-00370-8

    Obestatin promotes activation of NFTAc1. a Comparation of the effect of obestatin (10 nM) and insulin (1.72 µM) on utrophin, ß-dystroglycan, and α7-integrin protein expression on differentiating human DMD cells. b Analysis of pNFATc1(S172) and NFATc1 after obestatin (10 nM) or insulin (1.72 µM) administration on differentiating human DMD cells. c Representative immunofluorescence images of obestatin- or control-treated human DMD myotubes showing NFATc1 cellular location. Right panel , measurement of the number of nuclei showing NFATc1 location. Data are shown as mean ± SEM (* P < 0.05). d Representative images of vehicle- and obestatin-treated TAs showing NFATc1 expression. Right panel , measurement of the number of nuclei showing NFATc1 location. Data are shown as mean ± SEM (* P < 0.05). e Immunoblot analysis of PPP3 catalytic subunit (PPP3CA), pNFATc1(S172), NFATc1, utrophin, slow-MHC, fast-MHC, β-dystroglycan, α-syntrophin, NOS1, β1D-integrin, and α7-integrin in extracts of DMD cells transfected with control or PPP3CA siRNAs after obestatin treatment (10 nM). The inset shows Proposed model by which obestatin signalling triggers PPP3 to regulate the transcriptional activity of NFATc1. Data were expressed as mean ± SEM ( n = 3 per group; *, # P < 0.05)
    Figure Legend Snippet: Obestatin promotes activation of NFTAc1. a Comparation of the effect of obestatin (10 nM) and insulin (1.72 µM) on utrophin, ß-dystroglycan, and α7-integrin protein expression on differentiating human DMD cells. b Analysis of pNFATc1(S172) and NFATc1 after obestatin (10 nM) or insulin (1.72 µM) administration on differentiating human DMD cells. c Representative immunofluorescence images of obestatin- or control-treated human DMD myotubes showing NFATc1 cellular location. Right panel , measurement of the number of nuclei showing NFATc1 location. Data are shown as mean ± SEM (* P < 0.05). d Representative images of vehicle- and obestatin-treated TAs showing NFATc1 expression. Right panel , measurement of the number of nuclei showing NFATc1 location. Data are shown as mean ± SEM (* P < 0.05). e Immunoblot analysis of PPP3 catalytic subunit (PPP3CA), pNFATc1(S172), NFATc1, utrophin, slow-MHC, fast-MHC, β-dystroglycan, α-syntrophin, NOS1, β1D-integrin, and α7-integrin in extracts of DMD cells transfected with control or PPP3CA siRNAs after obestatin treatment (10 nM). The inset shows Proposed model by which obestatin signalling triggers PPP3 to regulate the transcriptional activity of NFATc1. Data were expressed as mean ± SEM ( n = 3 per group; *, # P < 0.05)

    Techniques Used: Activation Assay, Expressing, Immunofluorescence, Control, Western Blot, Transfection, Activity Assay

    Obestatin promotes activation of mitochondrial homeostasis via calcineurin and TFEB activity. a Immunoblot analysis of PPP3CA, PINK1, DRP1, FIS1, TFAM, PGC1a, OPA1, and MFN2 in extracts of DMD cells transfected with control or PPP3CA siRNAs after obestatin treatment (10 nM). b Immunoblot analysis of PPP3CA, pTFEB(S211), and TFEB in extracts of DMD cells transfected with control or PPP3CA siRNAs after obestatin treatment (10 nM). The inset shows the proposed model by which obestatin signalling triggers PPP3 to regulate the transcriptional activity of TFEB. c Analysis of nuclear translocation of TFEB after obestatin treatment (10 nM) in human DMD myotubes. In A-C, data were expressed as mean ± SEM ( n = 3 per group; *, # P < 0.05). d Proposed model by which obestatin signalling triggers PPP3, NFATc1 and TFEB activity to regulate mitochondrial homeostasis, autophagy, muscle fibber type specification and sarcolemma repair obestatin signalling under dystrophic conditions
    Figure Legend Snippet: Obestatin promotes activation of mitochondrial homeostasis via calcineurin and TFEB activity. a Immunoblot analysis of PPP3CA, PINK1, DRP1, FIS1, TFAM, PGC1a, OPA1, and MFN2 in extracts of DMD cells transfected with control or PPP3CA siRNAs after obestatin treatment (10 nM). b Immunoblot analysis of PPP3CA, pTFEB(S211), and TFEB in extracts of DMD cells transfected with control or PPP3CA siRNAs after obestatin treatment (10 nM). The inset shows the proposed model by which obestatin signalling triggers PPP3 to regulate the transcriptional activity of TFEB. c Analysis of nuclear translocation of TFEB after obestatin treatment (10 nM) in human DMD myotubes. In A-C, data were expressed as mean ± SEM ( n = 3 per group; *, # P < 0.05). d Proposed model by which obestatin signalling triggers PPP3, NFATc1 and TFEB activity to regulate mitochondrial homeostasis, autophagy, muscle fibber type specification and sarcolemma repair obestatin signalling under dystrophic conditions

    Techniques Used: Activation Assay, Activity Assay, Western Blot, Transfection, Control, Translocation Assay



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    human ppp3 catalytic subunit alpha  (Santa Cruz Biotechnology)
    92
    Santa Cruz Biotechnology human ppp3 catalytic subunit alpha
    Obestatin promotes activation of NFTAc1. a Comparation of the effect of obestatin (10 nM) and insulin (1.72 µM) on utrophin, ß-dystroglycan, and α7-integrin protein expression on differentiating human DMD cells. b Analysis of pNFATc1(S172) and NFATc1 after obestatin (10 nM) or insulin (1.72 µM) administration on differentiating human DMD cells. c Representative immunofluorescence images of obestatin- or control-treated human DMD myotubes showing NFATc1 cellular location. Right panel , measurement of the number of nuclei showing NFATc1 location. Data are shown as mean ± SEM (* P < 0.05). d Representative images of vehicle- and obestatin-treated TAs showing NFATc1 expression. Right panel , measurement of the number of nuclei showing NFATc1 location. Data are shown as mean ± SEM (* P < 0.05). e Immunoblot analysis of <t>PPP3</t> catalytic subunit (PPP3CA), pNFATc1(S172), NFATc1, utrophin, slow-MHC, fast-MHC, β-dystroglycan, α-syntrophin, NOS1, β1D-integrin, and α7-integrin in extracts of DMD cells transfected with control or PPP3CA siRNAs after obestatin treatment (10 nM). The inset shows Proposed model by which obestatin signalling triggers PPP3 to regulate the transcriptional activity of NFATc1. Data were expressed as mean ± SEM ( n = 3 per group; *, # P < 0.05)
    Human Ppp3 Catalytic Subunit Alpha, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+ppp3+catalytic+subunit+alpha/pmc12647412-285-11-17?v=Santa+Cruz+Biotechnology
    Average 92 stars, based on 1 article reviews
    human ppp3 catalytic subunit alpha - by Bioz Stars, 2026-07
    92/100 stars
    		  Buy from Supplier

    Image Search Results


    Obestatin promotes activation of NFTAc1. a Comparation of the effect of obestatin (10 nM) and insulin (1.72 µM) on utrophin, ß-dystroglycan, and α7-integrin protein expression on differentiating human DMD cells. b Analysis of pNFATc1(S172) and NFATc1 after obestatin (10 nM) or insulin (1.72 µM) administration on differentiating human DMD cells. c Representative immunofluorescence images of obestatin- or control-treated human DMD myotubes showing NFATc1 cellular location. Right panel , measurement of the number of nuclei showing NFATc1 location. Data are shown as mean ± SEM (* P < 0.05). d Representative images of vehicle- and obestatin-treated TAs showing NFATc1 expression. Right panel , measurement of the number of nuclei showing NFATc1 location. Data are shown as mean ± SEM (* P < 0.05). e Immunoblot analysis of PPP3 catalytic subunit (PPP3CA), pNFATc1(S172), NFATc1, utrophin, slow-MHC, fast-MHC, β-dystroglycan, α-syntrophin, NOS1, β1D-integrin, and α7-integrin in extracts of DMD cells transfected with control or PPP3CA siRNAs after obestatin treatment (10 nM). The inset shows Proposed model by which obestatin signalling triggers PPP3 to regulate the transcriptional activity of NFATc1. Data were expressed as mean ± SEM ( n = 3 per group; *, # P < 0.05)

    Journal: Molecular Biomedicine

    Article Title: Obestatin treatment links mitochondrial homeostasis and skeletal muscle repair in Duchenne muscle dystrophy

    doi: 10.1186/s43556-025-00370-8

    Figure Lengend Snippet: Obestatin promotes activation of NFTAc1. a Comparation of the effect of obestatin (10 nM) and insulin (1.72 µM) on utrophin, ß-dystroglycan, and α7-integrin protein expression on differentiating human DMD cells. b Analysis of pNFATc1(S172) and NFATc1 after obestatin (10 nM) or insulin (1.72 µM) administration on differentiating human DMD cells. c Representative immunofluorescence images of obestatin- or control-treated human DMD myotubes showing NFATc1 cellular location. Right panel , measurement of the number of nuclei showing NFATc1 location. Data are shown as mean ± SEM (* P < 0.05). d Representative images of vehicle- and obestatin-treated TAs showing NFATc1 expression. Right panel , measurement of the number of nuclei showing NFATc1 location. Data are shown as mean ± SEM (* P < 0.05). e Immunoblot analysis of PPP3 catalytic subunit (PPP3CA), pNFATc1(S172), NFATc1, utrophin, slow-MHC, fast-MHC, β-dystroglycan, α-syntrophin, NOS1, β1D-integrin, and α7-integrin in extracts of DMD cells transfected with control or PPP3CA siRNAs after obestatin treatment (10 nM). The inset shows Proposed model by which obestatin signalling triggers PPP3 to regulate the transcriptional activity of NFATc1. Data were expressed as mean ± SEM ( n = 3 per group; *, # P < 0.05)

    Article Snippet: To knockdown PPP3 expression in human DMD cells, siRNA specifically targeting human PPP3 catalytic subunit alpha (sc-36303, Santa Cruz Biotechnology, CA, US) was used.

    Techniques: Activation Assay, Expressing, Immunofluorescence, Control, Western Blot, Transfection, Activity Assay

    Obestatin promotes activation of mitochondrial homeostasis via calcineurin and TFEB activity. a Immunoblot analysis of PPP3CA, PINK1, DRP1, FIS1, TFAM, PGC1a, OPA1, and MFN2 in extracts of DMD cells transfected with control or PPP3CA siRNAs after obestatin treatment (10 nM). b Immunoblot analysis of PPP3CA, pTFEB(S211), and TFEB in extracts of DMD cells transfected with control or PPP3CA siRNAs after obestatin treatment (10 nM). The inset shows the proposed model by which obestatin signalling triggers PPP3 to regulate the transcriptional activity of TFEB. c Analysis of nuclear translocation of TFEB after obestatin treatment (10 nM) in human DMD myotubes. In A-C, data were expressed as mean ± SEM ( n = 3 per group; *, # P < 0.05). d Proposed model by which obestatin signalling triggers PPP3, NFATc1 and TFEB activity to regulate mitochondrial homeostasis, autophagy, muscle fibber type specification and sarcolemma repair obestatin signalling under dystrophic conditions

    Journal: Molecular Biomedicine

    Article Title: Obestatin treatment links mitochondrial homeostasis and skeletal muscle repair in Duchenne muscle dystrophy

    doi: 10.1186/s43556-025-00370-8

    Figure Lengend Snippet: Obestatin promotes activation of mitochondrial homeostasis via calcineurin and TFEB activity. a Immunoblot analysis of PPP3CA, PINK1, DRP1, FIS1, TFAM, PGC1a, OPA1, and MFN2 in extracts of DMD cells transfected with control or PPP3CA siRNAs after obestatin treatment (10 nM). b Immunoblot analysis of PPP3CA, pTFEB(S211), and TFEB in extracts of DMD cells transfected with control or PPP3CA siRNAs after obestatin treatment (10 nM). The inset shows the proposed model by which obestatin signalling triggers PPP3 to regulate the transcriptional activity of TFEB. c Analysis of nuclear translocation of TFEB after obestatin treatment (10 nM) in human DMD myotubes. In A-C, data were expressed as mean ± SEM ( n = 3 per group; *, # P < 0.05). d Proposed model by which obestatin signalling triggers PPP3, NFATc1 and TFEB activity to regulate mitochondrial homeostasis, autophagy, muscle fibber type specification and sarcolemma repair obestatin signalling under dystrophic conditions

    Article Snippet: To knockdown PPP3 expression in human DMD cells, siRNA specifically targeting human PPP3 catalytic subunit alpha (sc-36303, Santa Cruz Biotechnology, CA, US) was used.

    Techniques: Activation Assay, Activity Assay, Western Blot, Transfection, Control, Translocation Assay